Jaringan Laboratorium Medis

Vol. 6 No. 1 (2024): May 2024
Original Articles

The Effectiveness of Ylang Ylang Oil (Cananga Odorata) as a Deparaffinizing Agent in Hematoxylin-Eosin Staining

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  • Gela Setya Ayu Putri,
  • Dina Diyanah,
  • Arya Iswara

Published 2024-05-30

Keywords

  • Kenanga Oil,
  • Deparafinization,
  • HE Staining

Copyright (c) 2024 Jaringan Laboratorium Medis

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How to Cite

The Effectiveness of Ylang Ylang Oil (Cananga Odorata) as a Deparaffinizing Agent in Hematoxylin-Eosin Staining. (2024). Jaringan Laboratorium Medis, 6(1), 1-8. https://doi.org/10.31983/jlm.v6i1.10824

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Abstract

Histological staining usually requires deparaffinization before starting the staining process. Deparaffinization is a paraffin removal step that often uses a xylol solution. Deparaffinization with xylol has disadvantages, including being toxic, harmful to the human body, and hazardous to the environment. A safer alternative to xylol is required. Ylang-ylang oil, which contains β-caryophyllene, is non-polar and can be used as a substitute for xylol. This study aimed to compare the staining quality of guinea pig liver tissue slides with and without heating as a deparaffinization agent in hematoxylin-eosin (HE) staining. The research set up a quasi-experimental approach using samples of guinea pig liver tissue slides. The sample size was calculated using the Federer formula (n-1) (t-1) ≥ 15, resulting in a total sample of 27 slides divided into three treatment groups: xylol, ylang-ylang oil with and without heating. The Kruskal-Wallis test was used to examine the data. The quality staining of guinea pig liver slides deparaffinized with xylol was 100% good, and deparaffinization of ylang-ylang oil with heating was 100% good, whereas deparaffinization with ylang-ylang oil without heating was 11.1% less good, and the quality was good with an 88.9%. The normality test indicated that the data were not normally distributed (p<0.05). The Kruskal-Wallis test was then used.

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