Jaringan Laboratorium Medis

Vol. 1 No. 2 (2019): November 2019
Original Articles

Biochemistry Mycobacterium Tuberculosis Treated with Anti-Isoniazid Treatment

PDF
  • Elly Karlina,
  • Widodo,
  • Devi Etivia Purlinda
Elly Karlina
Poltekkes Kemenkes Semarang
Widodo
Poltekkes Kemenkes Semarang
Devi Etivia Purlinda
Poltekkes Kemenkes Semarang

Published 2023-11-28

Keywords

  • Mycobacterium tuberculosis,
  • Isoniazid,
  • Biochemistry

Copyright (c) 2019 Jaringan Laboratorium Medis

Creative Commons License

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

How to Cite

Biochemistry Mycobacterium Tuberculosis Treated with Anti-Isoniazid Treatment. (2023). Jaringan Laboratorium Medis, 1(2), 81-85. https://doi.org/10.31983/jlm.v1i2.5871

CrossMark



Dimensions

If it doesn't Appear, click here



PlumX Metrics



Impact Factor

or, Visit on here

Abstract

Mycobacterium tuberculosis exposed to Isoniazid experienced drug resistance due to morphological and selection factors, with Isoniazid concentrations of 0.5 μg / ml with a contact time of 24 hours, 48 hours, 72 hours, 96 hours, the results obtained in testing for NaCl 5% Kruskal wallis obtained sig 0.005 results there is a treatment effect on growth While in the semi-quantitative catalase test, a sig value of 0.007 was obtained with p <0.01 so it can be concluded that there is a significant influence between the duration of isoniazid exposure. resistance occurs due to the selection of strains that are resistant to treatment during the therapy process at 96 hours exposure with a percentage of 2%. In the exposure process it can be concluded that it will change the resistance to 5% NaCl, changes in Catalase test results and also causes strain selection that is resistant to isoniazid 0.5 µg / ml. 

PDF

Downloads

Download data is not yet available.

References

  1. Asmar, S., Sassi, M., Phelippeau, M., and Drancourt, M. 2016. Inverse correlation between salt tolerance and host-adaptation in mycobacteria. BMC research notes, 9(1), 1-9. https://doi.org/10.1186/s13104-016-2054-y
  2. Babady, N. E., and Wengenack, N. L. 2012. Clinical laboratory diagnostics for Mycobacterium tuberculosis, understanding tuberculosis—global experiences and innovative approaches to the diagnosis. ISBN, 978-953.
  3. Boum, Y., Orikiriza, P., Rojas-Ponce, G., Riera-Montes, M., Atwine, D., Nansumba, M., Bazira, J., Tuyakira,E., De Beaudrap, P., Bonnet, M., and Page, A. L. 2013. Use of colorimetric culture methods for detection of Mycobacterium tuberculosis complex isolates from sputum samples in resource-limited settings. Journal of Clinical Microbiology 51(7): 2273-2279. DOI: 10.1128/JCM.00749-13
  4. Conville, P. S., and Witebsky, F.G. 1998. Variables affecting results of sodium chloride tolerance test for identification of rapidly growing mycobacteria. Journal of Clinical Microbiology 36.6 : 1555-1559. DOI: 10.1128/JCM.36.6.1555-1559.1998
  5. Crofton, John, and D. A. Mitchison. 1948. Streptomycin resistence in pulmonary tuberculosis. British Medical Journal 2.4588 : 1009. doi: 10.1136/bmj.2.4588.1009
  6. Kochi, A., Vareldzis, B., and Styblo, K. 1993. Multidrug-resisten tuberculosis and its control. Research in microbiology, 144(2): 104-110. https://doi.org/10.1016/0923-2508(93)90023-U
  7. Larrouy-Maumus, G., Marino, L. B., Madduri, A. V., Ragan, T. J., Hunt, D. M., Bassano, L., Gutierrez, M.G., Moody, D.B., Pavan, F. R., and de Carvalho, L. P. S. 2016. Cell-Envelope Remodeling as a Determinant of Phenotypic Antibacterial Tolerance in Mycobacterium tuberculosis. ACS Infectious Diseases 2(5): 352-360. doi.org/10.1021/acsinfecdis.5b00148
  8. Ramaswamy, S. V., Reich, R., Dou, S. J., Jasperse, L., Pan, X., Wanger, A., Quitugua, T., and Graviss,A, 2003. Single nucleotide polymorphisms in genes associated with Isoniazid resistence in Mycobacterium tuberculosis. Antimicrobial Agents and Chemotherapy, 47 : 1241-1250. doi.org/10.1021/acsinfecdis.5b00148
  9. Widodo, W., Irianto, A., dan Pramono, H. 2017. Karakteristik Morfologi Mycobacterium tuberculosis yang Terpapar Obat Anti TB Isoniazid (INH) secara Morfologi. Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal, 33(3), 109-115. Doi 10.20884/1.mib.2016.33.3.316
  10. Zhang, Y., Garbe, T., and Young, D. 1993. Transformation with Kat G restores Isoniazid‐sensitivity in Mycobacterium tuberculosis isolates resisten to a range of drug concentrations. Molecular Microbiology, 8(3): 521-524. doi.org/10.1111/j.1365-2958.1993.tb01596.x
  11. Zhang, Y., Heym, B., Allen, B., Young, D., and Cole, S. 1992. The catalase—peroxidase gene and Isoniazid resistence of Mycobacterium tuberculosis, Nature, 358 (6387), 591 – 593. doi.org/10.1038/358591a0
  12. Zhang, Y., and Yew, W. W. 2009. Mechanisms of drug resistence in Mycobacterium tuberculosis [State of the art series. Drug-resisten tuberculosis. Edited by CY. Chiang. Number 1 in the series]. The International Journal of Tuberculosis and Lung Disease, 13(11): 1320-1330.