Jaringan Laboratorium Medis

Vol. 3 No. 1 (2021): May 2021
Original Articles

Histology of Mice (Mus Musculus) Liver Tissue Fixed with Carnoy's Solution With Variation of 4 Hours, 8 Hours and 12 Hours

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  • Arin Dwi Afrida,
  • Djoko Priyatno
Arin Dwi Afrida
Poltekkes Kemenkes Semarang
Djoko Priyatno
Poltekkes Kemenkes Semarang

Published 2023-12-04

Keywords

  • Microscopic Overview,
  • Mice Liver,
  • Fixation,
  • 10% NBF,
  • Carnoy's Solution

Copyright (c) 2021 Jaringan Laboratorium Medis

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How to Cite

Histology of Mice (Mus Musculus) Liver Tissue Fixed with Carnoy’s Solution With Variation of 4 Hours, 8 Hours and 12 Hours. (2023). Jaringan Laboratorium Medis, 3(1), 38-43. https://doi.org/10.31983/jlm.v3i1.7920

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Abstract

Histopathological examination is a routine examination process for each tissue in the anatomic pathology laboratory. The stages in making histology preparations are fixation stages. The type of solution that can be used as a fixative solution other than 10% NBF is Carnoy's solution. Carnoy's solution is a fixative solution with a relatively fast fixation process of about 1-4 hours. The advantages of Carnoy's solution can lyse erythrocytes and dissolve lipids, have the ability to maintain cell nuclei, and retain glycogen. The purpose of this study was to determine the histology of the liver tissue preparations of mice (Mus Musculus) which were fixed using Carnoy's solution with time variations of 4 hours, 8 hours, and 12 hours. This is an observational research with descriptive research design criteria. The results of the histology of the liver tissue preparations of mice (Mus Musculus) which were fixed using the 10% NBF solution group obtained 100% good preparations. In the Carnoy solution group with a time of 4 hours, it gave 2.2% results of poor preparations and 97.78% of good preparations. While the treatment group with Carnoy's solution with a time of 8 hours obtained as many as 11.11% of preparations that were not good and 88.89% of good preparations. and in the Carnoy solution group with a time of 12 hours, there were 44.44% of the poor preparations and 55.56% of the good preparations.

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References

  1. Ahmed, H. G., and Mohammed, A. I. I. (2011). A comparison study of histochemical staining of various tissues after Carnoy’s versus after formalin fixation. Journal of Cancer Science and Therapy, 3(4), 84–87. https://doi.org/10.4172/1948-5956.1000065
  2. Ariyadi, T. (2017). Kualitas Sediaan Jaringan Kulit Metode Microwave Dan Conventional Histoprocessing Pewarnaan Hematoksilin Eosin. Jurnal Labora Medika. 1 (1) : 7-11.
  3. Dias, A.R., Pereira, M.A., Mello, E.S., Zilberstein, B., Cecconello, I., Junior, U.R. (2014). Carnoy’s solution increases the number of examined lymph nodes following gastrectomy for adenocarcinoma: a randomized trial. Brazil : The International Gatric Cancer Association and The Japanese Gastric Cancer Association.
  4. Fajrina, S. N., Ariyadi, T., Nuroini, F., dan Semarang, U. M. (2018). Gambaran Kualitas Sediaan Jaringan Hati Menggunakan Larutan Fiksatif NBF 10 % dan Alkohol 70 % pada Pewarnaan HE (Hematoksilin-Eosin) dapat menyebabkan larutan fiksatif tidak menyerap dengan baik . Potongan jaringan yang diketahui bahwa metode fiksasi i. 1, 60–65.
  5. Jusuf, Ahmad A. (2009). Histoteknik Dasar. Skripsi. Fakultas Kedokteran Universitas Indonesia.
  6. Khristian, E., dan Inderiati, D. (2017). Sitohistoteknologi (N. Fitriana & H. Junianto (eds.); 2017th ed.). Pusat Pendidikan Sumber Daya Manusia Kesehatan.
  7. Lael, B. F., Sntosa, B., dan Aryadi, T. (2018). Perbedaan Penggunaan Xylol (Xylene) dan Toluol (Toluene) pada Proses Clearing terhadap Kualitas Preparat Awetan Permanen Cimex lectularius. Prosiding Seminar Nasional Mahasiswa Unimus, 1, 232–237.
  8. Miranti, I.P. (2010). Pengolahan Jaringan Untuk Penelitian Hewan Coba. Skripsi. Universitas Diponegoro.
  9. Musyarifah, Z., & Agus, S. (2018). Proses Fiksasi pada Pemeriksaan Histopatologik. Jurnal Kesehatan Andalas, 7(3), 443. https://doi.org/10.25077/jka.v7i3.900
  10. Nuralim, E. R., Rahayu, I. D., & Bekti, R. S. (2017). Comparative Analysis Of Fixation Using Carnoy Solution and Formalin Solution in Chicken Embryo ’ S Somites , Neural Tube , and Vascular Age Of 48 Hours With Hematoxylin-Eosin Staining Abstract Pendahuluan Embriologi merupakan ilmu yang sejak dari masa pem. 4.