Jaringan Laboratorium Medis

Vol. 3 No. 2 (2021): November 2021
Original Articles

Microscopic Profile of Mice Liver Tissue (Mus musculus) Fixed with Neutral Buffered Formalin (NBF 10%) and Helly Solution

Melly Agustin
Poltekkes Kemenkes Semarang

Published 2023-12-05

Keywords

  • Microscopic Picture,
  • Hepar Squeak,
  • NBF Fixation Solution 10%,
  • Helly Solution.

How to Cite

Microscopic Profile of Mice Liver Tissue (Mus musculus) Fixed with Neutral Buffered Formalin (NBF 10%) and Helly Solution. (2023). Jaringan Laboratorium Medis, 3(2), 90-95. https://doi.org/10.31983/jlm.v3i2.8053

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Abstract

The fixation solution that is widely used in anatomical pathology laboratories is NBF 10%, the excess of NBF 10% because the pH is close to neutral, can be stored in large quantities and a long time. Helly's fixation solution is a good fixation solution for the cytoplasm, and only requires 2-3 hours of fixation. Knowing the microscopic picture of the preparation of hepar mencit tissue (Mus musculus) fixated with NBF 10% and Helly solution. This research is an experimental study with a descriptive analysis approach. picture of hepar mencit tissue preparation (Mus musculus) fixation with NBF 10% obtained as much as 100% good preparation. While the fixated with Helly solution obtained as much as 66% good preparation. Conclusion: Microscopic picture of hepar mencit tissue preparation (Mus musculus) fixated with NBF is 10% better than Helly solution.

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References

  1. Afrianti, R., Ramadhani, P., Irsanti, P,N. (2017). Uji Efektivitas Ekstorgenik Ekstrak Etanol Jintan Hitam (Nigel Sativa L.) Terhadap Perkebangan Uterus Tikus Putih Betine. Jurnal Scientia, 7(8)
  2. Afrida, A. D., & Priyatno, D. (2021). Gambaran Histologi Jaringan Hepar Mencit ( Mus Musculus ) yang Difiksasi dengan Larutan Carnoy dengan Variasi Waktu 4 Jam , 8 Jam dan 12 Jam. Jaringan Laboratorium Medis, 03(01), 37–42.
  3. Alwi. M. A. (2016). Studi Awal Histoteknik: Fiksasi Dua Minggu Pada Gambaran Histologi Organ Ginjal, Hepar, Dan Pankreas Tikus Sprague Dawley Dengan Pewarnaan Hematoxylin-Eosin. Naskah Publikasi UIN Syarif Hidayatullah, Jakarta.
  4. Ariyadi,T., Suryono, H. (2017). Kualitas Sediaan Jaringan Kulit Metode Microwave dan Conventonal Histoprocessing Pewarnaan Hematoxylin Eosin. Jurnal Labora Medika, 1(1) 7-11.
  5. Elen,R,M. (2019). Gambaran Kualitas Mikroskopis Sediaan Hepar Mencit (Mus musculus) Dengan Pemotongan Ketebalan 2mm, 5mm, dan 8mm. KTI Poltekkes Kemenkes Semarang Jurusan Analis Kesehatan Diakses tanggal 28 November 2020, website:http://repository.poltekkessmg.ac.id/index.php?p= show_detail&id=20300&keywords=.
  6. Fajrina,N.S., Ariyadi, T& Nuroini, F. (2018). Gambaran Sediaan Jaringan Hati menggunakan Larutan Fiksatif NBF 10% dan Alkohol 70% pada Pewarnaan HE (Hematoxilin Eosin). Prosiding Seminar Nasional Mahasiswa Universitas Muhammadiyah, Vol (1).
  7. Jusuf, A. A. (2009). Histoteknik Dasar. Skripsi Fakultas Kedokteran Universitas Indonesia, Jakarta. Retrieved September 11 , 2020 from https://staff.ui.ac.id/
  8. Khristian, E., & Inderiati, D. (2017). Bahan Ajar Sitohistoteknologi Teknologi Laboratorium Medis (TLM) (1st ed.). Jakarta: BPPSDMK Kementerian Kesehatan.
  9. Miranti, Ika Pawitra. (2010). Pengolahan Jaringan Untuk Penelitian Hewan Coba. Skripsi Universitas Diponegoro, Semarang. Retrieved October 19, 2020 from http://www.m3undip.org.
  10. Musyarifah, Z., & Agus, S. (2018). Proses Fiksasi pada Pemeriksaan Histopatologik. Jurnal Kesehatan Andalas, 7(3), 443–453.
  11. Prasetiawan., et al (2016). Optimalisasi Automatic Slide Stainer Untuk Pewarnaan Jaringan Menggunakan Hematoxilin-Eosin. Universitas Negeri Jember . Diakses tanggal 11 Desember 2020.
  12. Pratiwi, E. N., & Armalina, D. (2021). Mikroskopis Preparat Mus Musculus Ginjal Dideparafinisasi dengan Minyak Zaitun pada Pewarnaan Eosin ( HE ) Hematoxylin ( HE ). Jaringan Laboratorium Medis, 03(01), 61–66.
  13. Sultana,F. (2011). Buku Ajar Histologi II. Semarang: Balai Penerbit Fakultas Kedokteran Univesitas Diponegoro.
  14. Suprianto, A. (2014). Perbandingan Efek Fiksasi Formalin Metode Intravital Dengan Metode Konvensional Pada Kualitas Gambaran Histologis Hepar Tikus. Naskah Publikasi Fakultas Kedokteran Universitas Tanjung Pura, Pontianak. Diakses tanggal 12 Desember 2020. Website:http://jurnal.untan.ac.id/index.php/jfk/article/vi ewFile/9042/8984