Jaringan Laboratorium Medis

Vol. 4 No. 2 (2022): November 2022
Original Articles

Microscopic Quality Profile of Mice Liver (Mus musculus) with 2µm, 5µm and 8µm Thickness Sections

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  • Mircha Restiana Elen
Mircha Restiana Elen
Poltekkes Kemenkes Semarang

Published 2023-12-07

Keywords

  • Microscopic,
  • Mice Liver,
  • Cutting Thickness

Copyright (c) 2022 Jaringan Laboratorium Medis

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How to Cite

Microscopic Quality Profile of Mice Liver (Mus musculus) with 2µm, 5µm and 8µm Thickness Sections. (2023). Jaringan Laboratorium Medis, 4(2), 71-78. https://doi.org/10.31983/jlm.v4i2.8483

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Abstract

The cutting stage is the network manufacturing stage which needs to be considered the thickness scale. The standard scale that is commonly used is 2-7 µm, while the thickness of 5 µm which is used as a standard for cutting the thickness of the liver tissue shows that the cell is good for cutting liver tissue in mice (Mus musculus). Mice have many advantages, while the liver organ is one of the organs that are often used in research. The aim of this study was to describe the microscopic quality of liver preparations of mice (Mus musculus) by cutting tissue thickness of 2 µm, 5 µm and 8 µm. This type of research includes experimental research with descriptive research criteria. The study design used a purposive sampling study approach. The results of the quality of microscopic images of mice tissue (Mus musculus) at a thickness of 2 µm there were 12% not good, 20% not good and 68% good. The thickness of 5 µm is 100% good, while the thickness of 8 µm is 8%, the preparation is not good,  36% is not good, and 56% is good. The conclusions of this study were microscopic tissue mice (Mus musculus) preparations with good quality 68%, 8 µm 56%, and 100% at a thickness of 5 µm.

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References

  1. Alwi, M. A. (2016). Studi Awal Histoteknik: Fiksasi 2 Minggu Pada Gambaran Histologi Organ Ginjal, Hepar, Dan Pancreas Tikus Sprague Dawley Dengan Pewarnaan Hematoxylin-Eosin. Jakarta: UIN Syarif Hidayatullah Jakarta. Diakses November 2018, http://repository.uinjkt.ac.id/dspace/bitstream/123456789/34236/1/MUHAMMAD%20AZHARAN%20ALWI-FKIK.pdf
  2. Ariyadi, T., Suryono, H. (2017). Kualitas Sediaan Jaringan Kulit Metode Microwave Dan Conventional Histoprocessing Pewarnaan Hematoxylin Eosin. Semarang: Jlabmed, 1 (1) 7-11, Diakses pada November 6, 2018, dari https://docobook.com/kualitas sediaan-jaringan-kulit-metodemicrowaved1cc3123246a95694cd6b020877efe7426404.html
  3. Hasanah U., Rusny, & Masri M. (2015). Analisis Pertumbuhan Mencit (Mus Musculusl.) ICR Dari Hasil Perkawinan Inbreeding Dengan Pemberian Pakan AD1 dan AD2. Jurusan Biologi, Fakultas Sains dan Teknologi, UIN Alauddin Makassar. Diakses pada Januari 9, 2019
  4. Julio E., Busman & Nurcahyani N. (2013). Struktur Histologis Hati Mencit (Mus Musculus L.) Sebagai Respon Terhadap Kebisingan. Lampung: Bandar Lampung, dari https://satek.unila.ac.id/wp-content/uploads/2014/03/4-127.pdf
  5. Khristian, E., Inderiati, D. (2017). Bahan ajar Teknologi Laboratorium Medis (TLM): Sitohistoteknologi. Cetakan pertama, Oktober.
  6. Matenaers, C., Popper B., Rieger A., Wanke1, R., & Blutke, A. (2018). Research Article: Practicable Methods For Histological Section Thickness Measurement In Quantitative Stereological Analyses. Diakses pada November 7, 2018, dari https://doi.org/10.1371/journal. pone.0192879
  7. Miranti I.P. (2010). Pengolahan Jaringan Untuk Penelitian Hewan Coba. Medical faculty of Diponegoro Univercity.
  8. Mulyono A., DH. Farida, Soesanti N.H. (2013). Histopatologi Hepar Tikus Rumah (Rattus Tanezumi) Infektif Patogenik Leptospira Spp. Jurnal Vektora, V (1), Diakses November, 2018. http://ejournal.litbang.depkes.go.id/index.php/vk/article/view/3332
  9. Ningrum D. I. L., & Dra. Abdulgani N., M.Si. (2014). Pengaruh Pemberian Ekstrak Ikan Gabus (Channa striata) pada Struktur Histologi Hati Mencit (Mus musculus) Hiperglikemik. http://www.digilib.its.ac.id/public/ITS-paper-41411-1510100045-paper.pdf
  10. Prahanarendra, G. (2015). Studi Awal Histoteknik: Gambaran Histologi Organ Ginjal, Hepar, Dan Pancreas Tikus Sprague Dawley Dengan Pewarnaan HE Dengan Fiksasi 3 Minggu. Jakarta: UIN Syarif Hidayatullah Jakarta. Diakses pada November 5, 2018, dari http://repository.uinjkt.ac.id/dspace/bitstream/123456789/29486/1/GALANG%20PRAHANARENDRA-FKIK.pdf
  11. Pramesti N.K.T., Wiratmini N,I., Astiti, N.P.A. (2017). Struktur Histologi Hati Mencit(Mus Musculus L.) Setelah Pemberian Ekstrak Daun Ekor Naga (Rhapidhophora Pinnata Schott). Bali:, dari https://www.researchgate.net/publication/320360954_STRUKTUR_HISTOLOGI_HATI_MENCITMus_musculus_L_SETELAH_PEMBERIAN_EKSTRAK_DAUN_EKOR_NAGA_Rhapidhophora_pinnata_Schott
  12. Pramono, S. (2012). Pengaruh Formalin Peroral Dosis Bertingkat Selama 12 Minggu Terhadap Gambaran Histopatologis Hepar Tikus Wistar. Semarang: Fakultas Kedokteran Universitas Diponegoro, dari http://eprints.undip.ac.id/37755/1/Ridha_Abdi_Wahab_G2A008154_Lap.KTI.pdf
  13. Prasetiawan E., Sabri E., & Ilyas S. (2012). Gambaran Histologis Hepar Mencit (Mus Musculus L.) Strain Ddw Setelah Pemberian Ekstrak N-Heksan Buah Andaliman (Zanthoxylum Acanthopodium Dc.) Selama Masa Pra Implantasi Dan Pasca Implantasi. Sumatera Utara. Diakses pada Januari 17, 2019, dari https://www.researchgate.net/publication/265905616_GAMBARAN_HISTOLOGIS_HEPAR_MENCIT_Mus_musculus_L_STRAIN_DDW_SETELAH_PEMBERIAN_EKSTRAK_N-HEKSAN_BUAH_ANDALIMAN_Zanthoxylum_acanthopodium_DC_SELAMA_MASA_PRA_IMPLANTASI_DAN_PASCA_IMPLANTASI
  14. Sumanto, D. (2017). Belajar Sitohistoteknologi Untuk Pemula. IAKIS (Ikatan Analis Kesehatan Indonesia Semarang)
  15. Suprianto A. (2014). Perbandingan Efek Fiksasi Formalin Metode Intravital dengan Metode Konvensional Pada Kualitas Gambaran Histologis Hepar Tikus. Pontianak, Diakses pada November 8, 2018, dari https://docobook.com/naskah-publikasi-perbandingan-efek-fiksasi-formalin-metode.html
  16. Setyawati A. (2015). Struktur Histologi Hati, Ginjal Dan Pankreas Mencit (Mus Musculus) Dengan Perlakuan Ekstrak Batang Akar Kuning (Fibraurea Tinctoria L.) Selama Organogenesis. Bogor: Institut Pertanian Bogor, dari https://id.scribd.com/document/358603784/pembuatan-preparat-histologi-ginjal-pdf
  17. Suvarna S. Kim, Layton C., & Bancroft JD. (2018). Theory and Practice of Histological Techniques. Eight Edition. China
  18. Yohana, W. (2017). Perbandingan Cairan Fiksasi Bouin Dengan Buffer Formalin Terhadap Hepar Tikus Putih. J Syiah Kuala Dent Soc, 2 (2), 97-101. Diakses pada November 11, 2018, dari www.jurnal.unsyiah.ac.id/JDS/article/download/9704/7656